The Olympic Delivery Authority (ODA) set out details of the investment in its new document "Pace", published yesterday .
The investment will go towards different DLR projects to expand its network for the 2012 Games, increase its number of rail cars and provide an even more reliable and frequent service.
The DLR’s current 67M passengers a year will benefit from these improvements as early as 2010, when all the projects will be completed.
ODA transport director Hugh Sumner said: "The DLR started in 1987 with 6M passengers. The fact that only 25 years later in 2012 it will be capable of carrying 100M passengers a year is testament to the crucial role it has played in regenerating east London."
Four new stations – Star Lane, Abbey Road, Stratford High Street and Stratford International – will be built along the Stratford International extension, which will open in July 2010. These new stations will give people who live in the area a better and more frequent connection to the Olympic Park and other London 2012 venues served by the DLR.