New members include Paralympic gold medal winner Dame Tanni Grey-Thompson, who brings experience of transport use by people with mobility problems.
Six other new appointments include representatives from the taxi and private hire industries. London Council's Transport and Environment Committee chairman Daniel Moylan has been appointed to strengthen links with the capital’s boroughs.
Johnson said: "I am thrilled that we have been able to assemble a board of quite staggering expertise. Each and every member is chock full of the ideas, enthusiasm and energy that will propel the improvements we intend to make to the capital’s public transport system; especially to make it easier and safer to walk and cycle.
"Every member of the board knows that the decisions they take will potentially impact on millions of lives. There are eight million experts on getting round London living in our city and their opinions will be crucial in helping us provide the capital with a transport system that is safe, pleasant and easy to use."
The six new members of the board are Dame Tanni Grey-Thompson, Bob Oddy, Steve Wright, Keith Williams, Charles Belcher and Daniel Moylan.
Three members of the board - Tim Parker, Kulveer Ranger and Steven Norris - had previously been appointed by the Mayor. Seven previous board members have been reappointed. They are Christopher Garnett, Tony West, Eva Lindholm, Mike Hodgkinson, Patrick O’Keeffe, Judith Hunt and Peter Anderson.