The scheme will drive down the quantity of both CO2 and particulate emissions, and reduce congestion on the road.
Between 110 and 176 million lorry miles would be removed from the roads each year.
The plan aims to drive freight up, in parallel with passenger numbers.
It allows for rail freight traffic to increase alongside growing passenger demand.
Ian Brown, MD London Rail said, "The rail strategy is part of a multi modal plan for addressing the freight objectives in the London Plan and the Mayors Transport Strategy.
"It was developed in liaison with rail industry partners, and provides the basis of TfL's recommendations for investment in rail freight. "
The plan shows how freight will grow up to 2016.
Read the strategy at www.tfl.gov.uk/businessandpartners/4927.aspx