Slough Borough Council has made a formal objection against plans to build a railway depot in Langley to facilitate the £43bn High Speed 2 project.
The Berkshire local authority submitted a petition against the proposal to the Parliamentary committee formed to hammer out the detail of the route for phase one of the rapid rail link.
Promoter HS2 Ltd plans to build a Heathrow Express train depot north-east of Langley station so it can demolish the existing facility at Old Oak Common to make way for a major HS2 station.
Slough Borough Council assistant director of assets, infrastructure and regeneration Joe Carter said: “We’ve got serious concerns about the impact of the depot, both while it’s being constructed and when it becomes fully operational.
“Major issues like road access, air quality and the loss of land earmarked for much-needed family housing have not been properly considered, and no details have been given on how the effects would be mitigated.
“Businesses might decide not to invest in the area, and the move could affect plans to build a western rail link to Heathrow.”
The petition called for the depot to move to North Pole East in London, another site along the Heathrow Express line, or further east at Langley.
A HS2 Ltd spokesperson said: “It is important that all parties who feel they may be affected by the construction and operation of HS2 are able to petition.
“These petitions will now be considered by the select committee as part of the hybrid bill parliamentary process.”