Aberdeenshire Council's Infrastructure Services Committee yesterday refused outline planning permission for Trump's golf resort at Balmedie, Aberdeenshire.
The American billionaire, who is the face of the US version of The Apprentice, had won outline planning approval last week, now overturned by the Council vote.
Trump International Golf Links (Scotland) had planned a £1bn development along a spectacular stretch of coast in north eastern Scotland.
The council debated the issue for three hours before voting, and eventually tying, at seven votes for and seven against.
The casting vote went to the committee chair, Councillor Martin Ford, who took the decision to refuse permission.
The Trump organisation will now have to consider whether or not to appeal the decision to Scottish ministers.